The role of IKK-2/NF-κB signaling cascade in the activation of peritoneal macrophages by humic acids of peat

Introduction . Macrophages occupy a special place in the formation and coordination of immune response, their population is characterized by heterogeneity, however, two main types are distinguished among them: pro-inflammatory (classically activated) macrophages M1, which activated interferon-γ (IFN-γ) or lipopolysaccharide (LPS) bacteria, produce cytokines, nitric oxide (NO) initiate an anti-inflammatory (alternatively M2, such cytokines as interleukin 1 (IL-1), IL-10 IL-13 associated with suppression response tissue healing. The proinflammatory properties most often activation intracellular signaling cascade participation IKK-2 kinase complex nuclear factor-κB (NF-κB), important signal transduction molecule. Previously, authors showed that humic acids (HA) peat cause appearance macrophages; mechanisms have not been studied. Aim Study contribution IKK-2/NF-κB to peat. Materials methods studies were carried out using cultural western blotting. Results discussion It was shown addition inhibitor macrophage culture reduced ability HA stimulate production cells. Western blotting revealed after 30 minutes simultaneous incubation macrophages, content NF-κB p65 molecule cells increased, 60 it decreased values intact control, 17 hours 3 times compared control level. However, 30-minutes did blocking effect on IKK kinase, whereas hour co-incubation substances, decrease level observed. This trend continued hours. Conclusion. stimulating realized, other things, due further release subunit dependence degree period interaction cell activating agents traced. start factor already within first minutes.